ELISA - Quantitative
  • Load image into Gallery viewer, ELISA - Quantitative

Description
Features
Downloads
Media

Description

How the Test Works
AgraQuant® Mycotoxin products are direct competitive enzyme-linked immunosorbent assays (ELISA). One universal sample extraction using a 70% methanol solution can be applied to analyse six classes of mycotoxins with eight different kits. Some kits apply a dilution of the sample extract to reduce matrix effects.

After combining sample extract or calibrators and enzyme conjugated toxin in the antibody coated well the competitive reaction takes 10 – 60 min (first incubation). Subsequent washing removes unbound reactants followed by the addition of substrate starting a colour reaction that takes 5 – 20 min (second incubation). The intensity of the colour is inversely proportionate to the concentration of mycotoxin in the sample or standard. A stop solution prevents further development of the colour reaction enabling reproducible measurements of the intensity using a microwell reader. Results can be interpreted by using Romer Labs spreadsheets.

 

Kit Contents
Each kit comes complete with 5 to 6 standards, 48 or 96 antibody- coated microwells, 48 or 96 colour- coded dilution microwells, conjugate, substrate and stop solution.

Features

Easy

  • One sample extraction to analyse six mycotoxins
  • Simple workflow and no clean up steps required

 

Convenient

  • Wide range of validated commodities
  • Up to 30 minutes reading time after stopping the reaction

 

Cost-effective

  • 48 or 96 breakaway microwell format
  • Minimises waste and maximizes value

 

Media

ELISA - Quantitative